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. CRK12:CYC9 interact in a very yeast two-hybrid assay and kind an Lively protein kinase advanced in procyclic and bloodstream variety T. bruceiTo research the subcellular localization of your CRK12 protein, a pEarleyGate104 vector was utilized for a transient expression with the CRK12 protein fused to yellow fluorescent protein (YFP). The confocal